您是(shi)否希望懸(xuan)浮(fu)細(xi)(xi)胞(bao)的免疫熒光像貼壁細(xi)(xi)胞(bao)一樣容易?現在是(shi)這(zhe)樣!Shi-fix™(由Shikhar生物技術公司制造)蓋滑允許懸(xuan)浮(fu)細(xi)(xi)胞(bao)分(fen)層或直(zhi)接作為單層生長(chang)。簡單,無(wu)憂的實驗方(fang)案(an),無(wu)需離心柱即(ji)可將懸(xuan)浮(fu)細(xi)(xi)胞(bao)固(gu)定到載(zai)玻片(pian)上。只(zhi)需將細(xi)(xi)胞(bao)添加到蓋玻片(pian)中(zhong),等待30分(fen)鐘,用PBS洗(xi)滌(di)未結合的細(xi)(xi)胞(bao)并(bing)繼續(xu)免疫染(ran)色(或繼續(xu)培養以獲得所需的細(xi)(xi)胞(bao)密(mi)度(du))。
這(zhe)些(xie)蓋玻片(pian)(pian)也可用于染(ran)色懸(xuan)浮細(xi)胞(bao)(bao)以(yi)進行顯微鏡檢(jian)查,或用于固定懸(xuan)浮細(xi)胞(bao)(bao)以(yi)進行共聚焦顯微鏡檢(jian)查。更重(zhong)要的是,如果需要,您還可以(yi)在(zai)細(xi)胞(bao)(bao)附著在(zai)蓋玻片(pian)(pian)上時刺激它們。您可以(yi)像(xiang)貼(tie)(tie)壁細(xi)胞(bao)(bao)一樣處理非(fei)貼(tie)(tie)壁細(xi)胞(bao)(bao)!
Do you wish immunofluorescence of suspension cells was as easy as with adherent cells? Now it is! Shi-fix™ (manufactured by Shikhar Biotech) cover-slips allow suspension cells to be layered on them or directly grown as a monolayer. Easy, hassle-free protocol and no spin columns needed for fixing suspension cells to slides. Just add your cells to the coverslips, wait for 30 mins, wash unbound cells with PBS and proceed to immunostaining (or continue the culture to obtain desired cell densities).
These cover-slips can also be used for staining suspension cells for microscopy, or for fixing suspension cells for confocal microscopy. What’s more, you can also stimulate the cells while they are attached on the cover-slip, should you need to do so. You can work on non-adherent cells as if they were adherent!
SHI-FIX cover-slips玻片做的懸浮細胞免疫熒光實驗圖:
SHI-FIX cover-slips玻片的操作步驟:簡單!方便!!
SHI-FIX cover-slips玻片的產品訂購信息: